Human Papillomavirus (HPV) detection in plasma and in peripheral blood mononuclear cells (PBMC) of women with a recent history of cervical dysplasia.
PHE ePoster Library. Cocuzza C. Apr 10, 2019; 257521; 15442
Clementina Cocuzza
Clementina Cocuzza
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Abstract
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Abstract Background:
Previous studies have associated the presence of HPV DNA in the bloodstream of women with cervical cancer with a greater risk of recurrences or metastasis. Less is known regarding HPV DNA in blood of women with precancerous lesions. The aim of this pilot study is to investigate the presence of HPV nucleic acids in cervical, plasma and PBMCs samples of 100 women with a recent history of cervical dysplasia.
Methods:
Paired blood and cervical samples have been collected from 53 women referred to colposcopy at San Gerardo Hospital, Monza, Italy. Nucleic acids extraction was performed using NucliSENS easyMAG (bioMérieux). HPV detection in cervical samples was assessed by real-time PCR using AnyplexII™ HPV28 (Seegene). HPV 16, 18, 31, 33, 45, 51 and 52 DNA detection on plasma and PBMC was performed using quantitative 'in house' genotype specific real-time PCR assays. Genotype-specific oncogenic transcripts detection was assessed by “in house” real-time RT-PCR assays using iTaq™ Universal SYBR ® Green One-Step Kit..
Results:
One or more HPV types were detected in 83% (44/53) of cervical samples, with HPV16 and HPV31 being the most prevalent genotypes. Seven women (7/53; 13.2%) were found to be HPV DNA positive in plasma samples with HPV16 being the most prevalent genotype showing an average viral load of 336 copies/ml. HPV detection in PBMCs showed a positivity of 4% (2/53), both for HPV16 with a viral load of 6.87E+01 and 1.32E+01 copies/105 cells respectively. One of these samples was also positive for HPV16 oncogenic transcripts. ConclusionsPreliminary results confirm that HPV can be detected in peripheral blood samples of women with a recent history of cervical dysplasia. Further studies are required to evaluate the significance and the possible consequences of the presence of HPV DNA and RNA in the bloodstream of women with early stages of cervical dysplasia.
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