New strategies in cervical cancer screening: evaluation of HPV-testing on vaginal and first-void urine self-samples versus clinician-collected cervical samples
PHE ePoster Library. Martinelli M. Apr 9, 2019; 257514; 15425
Marianna Martinelli
Marianna Martinelli
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Abstract Background:
Self-sampling has been shown to be a cost-effective method to increase cervical cancer screening participation among hard-to-reach women, through high-risk Human Papillomavirus (HPV) detection. The aim of this study was to evaluate the accuracy of self-sampling as compared to physician-collected cervical samples in women attending the colposcopy clinic with a recent diagnosis of cervical dysplasia. Materials/methods:Self-collected vaginal samples using FLOQSwabs™ (Copan), first-void urines collected using Colli-Pee (Novosanis) and physician administered cervical samples were obtained from women referred for colposcopy. Nucleic acids were isolated by NucliSENS easyMAG (bioMérieux) and HPV detection was carried out using AnyplexII HPV28 (Seegene). Sample adequacy through cellularity assessment (quantification of human CCR5 gene) and HPV-16, 18, 31, 33, 45, 51 and 52 genotype-specific viral loads were evaluated by means quantitative real-time PCR. To evaluate the self-sampling acceptability, participants were asked to complete a questionnaire..
:Preliminary data from 53 patients showed an adequate sample cellularity for all samples (mean value for urine, vaginal and cervical samples: 2.09E+06, 2.07E+06 and 3.16E+06 cells/sample, respectively). Concordant high-risk HPV (hrHPV) detection for at least one type was demonstrated in 90% of urine and in 97% of vaginal self-collected. HPV16 resulted the most frequently hrHPV type detected, with the highest viral loads, followed by HPV31 and HPV68. Infections with multiple HPV types were shown in 45% of women. 83.3% of patients reported to prefer self-sampling to physician-collected sampling. Conclusion:HPV detection using in self and clinician-collected samples showed a high degree of concordance. These data demonstrate promising results for the use of urine and vaginal self-collected samples in cervical cancer screening programs.
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