Use of a phage model to determine efficacy of a BSL-4 disinfectant under realistic in-use conditions
PHE ePoster Library. Richardson S. Apr 9, 2019; 257485; 15347
Mr. Samuel Richardson
Mr. Samuel Richardson
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Abstract Biosafety Level 4 (BSL-4) encompasses the most stringent biocontainment procedures, reserved for a handful of human viruses that pose the greatest health risk to personnel. PHE is in the process of designing the UK's first suited BSL-4 laboratory. As part of this process the laboratory's exit procedure, which involves a chemical decontamination shower of the suits, is being validated.This study makes use of enveloped bacteriophage Phi 6, an Ebola virus surrogate, to assess efficacy of Micro-Chem Plus™ (MCP), a widely-used BSL-4 disinfectant. Disinfection tests were performed in suspension and on surface-dried virus using methods adapted from standards EN 14476:2005 and ASTM E2197. Surface testing was performed on representative coupons (2cm2) of BSL-4 suit material to simulate a 10µl droplet of contamination, in 'clean' (0.3 g/l BSA) and 'dirty' (3 g/l BSA + erythrocytes) conditions. Log10 reduction values were determined for 5 % MCP against original starting titre (suspension testing) and virus titre recovered from coupons dried for 5 hours (surface testing).5% MCP showed potent virucidal activity in suspension, generating consistent >6 log10 reductions even after storage for 18 weeks and in the presence of 'dirty' soil conditions. Disinfectant efficacy was lower in surface tests; materials from the main body, zip edges and visor of a PVC-coated fabric suit and nitrile glove material generated log10 reductions between 3.9 and 4.8 for 'clean' inocula. 'Dirty' conditions yielded higher variability, with reductions impaired by up to 2 log10 in some tests. Preliminary results for material from a chlorinated polyethylene suit suggest higher log10 reductions (clean = 6.4, dirty = 4.8).In conclusion, both surface material and levels of organic matter can affect MCP surface efficacy. Nevertheless, tests currently indicate that MCP shows suitable efficacy for use as a chemical disinfectant in the new suited BSL-4 facility. Funding Funding was received from PHE.
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