Abstract Pharmacy flu vaccinations within the East of England (EoE) were approved by the NHS on the 20thJuly 2015, to increase uptake in at risk patients. The Pharmaceutical services negotiating committee negotiated a recharge to the NHS of £10.30 per vaccination (£1.50 per vaccine more than GP's). Concerns were raised of pharmacies only tapping into the GP's susceptible populations, and being responsible for the low uptake observed in some practices. In response to these concerns an analysis of Immform and Pharmoutcomes data for each CCG within the EoE was collated to look at the trends of pharmacy vaccination on overall uptake and isolate the eligibility groups in most need of attention per region and CCG.The analysis found that vaccination uptake reduced across the EoE over the 3 years of available data. Evidence of data discrepancies, however was found resulting in the increased the total population eligible likely caused by the addition of pharmacy vaccinations. The total number and pharmacy derived vaccinations increased year upon year while GP vaccinations decreased, providing evidence of pharmacies taking from the GPs susceptible population.When looking at the lowest preforming GP practices (>60% of a practices eligible vaccinated population vaccinated by other providers), it was found that only a small number had >10% of their eligible population vaccinated by pharmacies. In contrast many practices lost >10% of their registered eligible population to Other vaccine providers.The eligibility groups displaying the lowest vaccine uptake using ImmForm data are Obese (26%), Asplenia (35%) and Carers (37%) for the influenza season 2016-2017. The carer eligibility is of particular interest due to its numbers of pharmacy vaccinations doubling every year, suggesting pharmacies may have begun to target this group. This is a promising trend especially for an eligible group with a historically low uptake.